Generator
Part:BBa_K566030:Design
Designed by: Daniel Rodriguez Group: iGEM11_UANL_Mty-Mexico (2011-09-27)
CcaS gene (constitutive expression)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1299
Illegal SpeI site found at 1394 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1299
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 670
Illegal SpeI site found at 1394 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1299
Illegal XhoI site found at 2316 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1299
Illegal SpeI site found at 1394 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1299
Illegal SpeI site found at 1394
Illegal AgeI site found at 1334 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Due to illegal sites it was implemented UANLBricks assambly
Source
UANLBricks assambly
References
1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.